In vitro anthelmintic activity of Chenopodium ambrosioides for control of Neoechinorhyncus buttnerae in tambaqui (Colossoma macropomum)

Neoechinorhyncus buttnerae is among the main causative agents of diseases in tambaqui (Colossoma macropomum) and has caused great losses fish farming. Although important, there still no effective treatment protocol for acanthocephalosis. Therefore, aim this study was to evaluate vitro anthelmintic activity Chenopodium ambrosioides (mastruz) control buttnerae. Parasites were collected from intestine naturally infected animals randomly distributed Petri dishes containing following treatments triplicate standardized 5 mL RPMI1640 medium: TR1 (RPMI+spray-dried extract mastruz 10%); TR2 5%); positive (RPMI+levamisole 15 mg/mL); negative (RPMI). The parasites kept at room temperature observed every min, considered dead when they remained motionless even after external stimuli. Body deformities exposure (TR1 TR2), both with spray-dried controlling acanthocephalans first min experiment. been shown be an alternative N. potential continuing vivo studies developing a therapeutic protocol.